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Synaptotagmin-1 Polyclonal antibody

Name: Synaptotagmin-1 Polyclonal antibody
Brand: Proteintech
SKU: 14511-1-AP

Synaptotagmin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14511-1-AP

Synonyms

Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCIF

WB analysis using 14511-1-AP
mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HCT 116 using 14511-1-AP
HCT 116 (WT and SYT1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 14511-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
WB analysis of human liver using 14511-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 14511-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14511-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HCT 116 using 14511-1-AP
HCT 116 lysates prepared and IP of SYT1 performed using 1.0 μg of 14511-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of mouse testis using 14511-1-AP
IP result of anti-Synaptotagmin-1 (IP:14511-1-AP, 4ug; Detection:14511-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IHC staining of rat small intestine using 14511-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14511-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HCT 116 using 14511-1-AP
HCT 116 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 14511-1-AP at 1:500 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Cat No. 14511-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse cerebellum tissue, HCT 116 cells, mouse testis tissue, human liver tissue, Y79 cells
Positive IP detected in	mouse testis tissue, HCT 116 cells
Positive IHC detected in	rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HCT 116 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:4000-1:20000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 15 publications below
IHC	See 3 publications below
IF	See 5 publications below
IP	See 1 publications below

Product Information

14511-1-AP targets Synaptotagmin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synaptotagmin-1 fusion protein Ag5970 相同性解析による交差性が予測される生物種
Full Name	synaptotagmin I
Calculated molecular weight	48 kDa
Observed molecular weight	65 kDa, 47 kDa
GenBank accession number	BC058917
Gene symbol	Synaptotagmin-1
Gene ID (NCBI)	6857
RRID	AB_2199166
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-1 antibody 14511-1-AP	Download protocol
IHC protocol for Synaptotagmin-1 antibody 14511-1-AP	Download protocol
IF protocol for Synaptotagmin-1 antibody 14511-1-AP	Download protocol
IP protocol for Synaptotagmin-1 antibody 14511-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Rep The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus Authors - Lang Wen View Article
human	WB	Theranostics ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells. Authors - Tianhui Pan View Article
		Front Mol Neurosci A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13. Authors - Yun Li View Article
mouse	WB,IHC	J Psychiatr Res Early life stress enhances the susceptibility to depression and interferes with neuroplasticity in the hippocampus of adolescent mice via regulating miR-34c-5p/SYT1 axis Authors - Shuwen Yu View Article
mouse	IHC,WB	Biochim Biophys Acta Mol Cell Biol Lipids Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet. Authors - Sara Sancho-Knapik View Article
mouse	WB	J Proteomics Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome. Authors - Hao-Long Zeng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-1 Polyclonal antibody

Synaptotagmin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14511-1-AP

WB analysis of HCT 116 using 14511-1-AP

WB analysis of human liver using 14511-1-AP

WB analysis of Y79 using 14511-1-AP

WB analysis of mouse testis using 14511-1-AP

IP experiment of HCT 116 using 14511-1-AP

IP experiment of mouse testis using 14511-1-AP

IHC staining of rat small intestine using 14511-1-AP

IF Staining of HepG2 using 14511-1-AP

IF Staining of HCT 116 using 14511-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus

ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells.

A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.

Early life stress enhances the susceptibility to depression and interferes with neuroplasticity in the hippocampus of adolescent mice via regulating miR-34c-5p/SYT1 axis

Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.