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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-11 Polyclonal antibody

Synaptotagmin-11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12031-1-AP

Synonyms

KIAA0080, Synaptotagmin 11, synaptotagmin XI, Synaptotagmin-11, SYT11, SytXI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-11 Polyclonal antibody (12031-1-AP)

    WB analysis using 12031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Synaptotagmin-11 Polyclonal antibody (12031-1-AP)

    WB analysis of mouse skeletal muscle using 12031-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-11 Polyclonal antibody (12031-1-AP)

    IHC staining of mouse brain using 12031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-11 Polyclonal antibody (12031-1-AP)

    IHC staining of mouse brain using 12031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12031-1-AP (Synaptotagmin-11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inrat brain tissue, mouse skeletal muscle tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

12031-1-AP targets Synaptotagmin-11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptotagmin-11 fusion protein Ag2663 相同性解析による交差性が予測される生物種
Full Name synaptotagmin XI
Calculated molecular weight 431 aa, 48 kDa
Observed molecular weight 48 kDa，96 kDa
GenBank accession numberBC013690
Gene symbol Synaptotagmin-11
Gene ID (NCBI) 23208
RRIDAB_10638432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-11 antibody 12031-1-APDownload protocol
IHC protocol for Synaptotagmin-11 antibody 12031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biochem Genet

Constructing a Novel Amino Acid Metabolism Signature: A New Perspective on Pheochromocytoma Diagnosis, Immune Landscape, and Immunotherapy

Authors - Zechen Yan
View Article