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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-12 Polyclonal antibody

Synaptotagmin-12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55015-1-AP

Synonyms

SRG1, Synaptotagmin 12, synaptotagmin XII, Synaptotagmin-12, SYT12, SytXII

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using Synaptotagmin-12 Polyclonal antibody (55015-1-AP)

    WB analysis of mouse brain using 55015-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Synaptotagmin-12 Polyclonal antibody (55015-1-AP)

    WB analysis of SH-SY5Y using 55015-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Synaptotagmin-12 Polyclonal antibody (55015-1-AP)

    WB analysis of mouse brain using 55015-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Synaptotagmin-12 Polyclonal antibody (55015-1-AP)

    WB analysis of SH-SY5Y using 55015-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55015-1-AP targets Synaptotagmin-12 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name synaptotagmin XII
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberNM_177963
Gene symbol Synaptotagmin-12
Gene ID (NCBI) 91683
RRIDAB_10859791
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYT12 belongs to the synaptotagmin family. It may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. The antibody is specific to STY12.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-12 antibody 55015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins.

Authors - Yingchao Li
View Article
mouseWB,IF

Nat Neurosci

Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1

Authors - Hongfei Xu
View Article