Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human kidney using 15413-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15413-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15413-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 15413-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15413-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15413-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15413-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15413-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 15413-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15413-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15413-1-AP
IP result of anti-SYT17 (IP:15413-1-AP, 4ug; Detection:15413-1-AP 1:800) with mouse brain tissue lysate 6000ug.
IHC staining of human brain using 15413-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 15413-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 15413-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 15413-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 15413-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYT17 antibody (15413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

SYT17 Polyclonal antibody

SYT17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15413-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

synaptotagminXVII, Synaptotagmin-17, Synaptotagmin17, Synaptotagmin XVII, Synaptotagmin 17

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human kidney tissue, A375 cells, human brain tissue, human testis tissue, mouse brain tissue, mouse lung tissue, PC-3 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human brain tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

15413-1-AP targets SYT17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SYT17 fusion protein Ag7691 相同性解析による交差性が予測される生物種
Full Name	synaptotagmin XVII
Calculated molecular weight	54 kDa
Observed molecular weight	75-80 kDa
GenBank accession number	BC004518
Gene Symbol	SYT17
Gene ID (NCBI)	51760
RRID	AB_2199457
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BSW7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SYT17, or synaptotagmin 17, is a protein that belongs to the synaptotagmin family, which is known for its role in regulating exocytosis at the plasma membrane. Reports find high levels of expression in hippocampus, particularly in pyramidal cell layers and in cultured hippocampal neurons. SYT17 is thus a multifunctional regulator of intracellular protein trafficking in excitatory hippocampal neurons, modulating both neural development and synaptic physiology. (PMID: 31387992, PMID: 36332665)

Protocols

Product Specific Protocols
WB protocol for SYT17 antibody 15413-1-AP	Download protocol
IHC protocol for SYT17 antibody 15413-1-AP	Download protocol
IF protocol for SYT17 antibody 15413-1-AP	Download protocol
IP protocol for SYT17 antibody 15413-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC,IF	Int Immunol Increased urinary exosomal SYT17 levels in chronic active antibody-mediated rejection after kidney transplantation via the IL-6 amplifier. Authors - Yusuke Takada KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human kidney using 15413-1-AP

WB analysis of mouse brain using 15413-1-AP

WB analysis of mouse brain using 15413-1-AP

WB analysis of A375 using 15413-1-AP

WB analysis of PC-3 using 15413-1-AP

WB analysis of PC-3 using 15413-1-AP

WB analysis of mouse lung using 15413-1-AP

WB analysis of human brain using 15413-1-AP

WB analysis of A375 using 15413-1-AP

WB analysis of human testis using 15413-1-AP

IP experiment of mouse brain using 15413-1-AP

IHC staining of human brain using 15413-1-AP

IHC staining of human lung cancer using 15413-1-AP