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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-4 Polyclonal antibody

Synaptotagmin-4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20472-1-AP

Synonyms

HsT1192, KIAA1342, Synaptotagmin 4, synaptotagmin IV, Synaptotagmin-4, SYT4, SytIV

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using Synaptotagmin-4 Polyclonal antibody (20472-1-AP)

    WB analysis of SH-SY5Y using 20472-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20472-1-AP (Synaptotagmin-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Synaptotagmin-4 Polyclonal antibody (20472-1-AP)

    WB analysis of mouse brain using 20472-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20472-1-AP (Synaptotagmin-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Synaptotagmin-4 Polyclonal antibody (20472-1-AP)

    WB analysis of A431 using 20472-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20472-1-AP (Synaptotagmin-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inSH-SY5Y cells, A431 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20472-1-AP is Synaptotagmin-4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptotagmin-4 fusion protein Ag14318 相同性解析による交差性が予測される生物種
Full Name synaptotagmin IV
Calculated molecular weight 425 aa, 48 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC036538
Gene symbol Synaptotagmin-4
Gene ID (NCBI) 6860
RRIDAB_10695633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-4 antibody 20472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols