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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-6 Polyclonal antibody

Synaptotagmin-6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17488-1-AP

Synonyms

Synaptotagmin 6, synaptotagmin VI, SYT6, SytVI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-6 Polyclonal antibody (17488-1-AP)

    WB analysis using 17488-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17488-1-AP (Synaptotagmin-6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Synaptotagmin-6 Polyclonal antibody (17488-1-AP)

    IHC staining of human liver using 17488-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17488-1-AP (Synaptotagmin-6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SYT6 Polyclonal antibody (17488-1-AP)

    IHC staining of human liver using 17488-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17488-1-AP (SYT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17488-1-AP targets Synaptotagmin-6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptotagmin-6 fusion protein Ag11575 相同性解析による交差性が予測される生物種
Full Name synaptotagmin VI
Calculated molecular weight425aa,48 kDa; 510aa,57 kDa
Observed molecular weight70 kDa
GenBank accession numberBC044948
Gene symbol Synaptotagmin-6
Gene ID (NCBI) 148281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-6 antibody 17488-1-APDownload protocol
IHC protocol for Synaptotagmin-6 antibody 17488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols