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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

SYTL2 Polyclonal antibody

SYTL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12359-1-AP

Synonyms

CHR11SYT, Exophilin 4, KIAA1597, SGA72M, SLP2, SLP2A, synaptotagmin like 2, Synaptotagmin like protein 2, SYTL2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYTL2 Polyclonal antibody (12359-1-AP)

    WB analysis using 12359-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12359-1-AP (SYTL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SYTL2 Polyclonal antibody (12359-1-AP)

    WB analysis of Jurkat using 12359-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12359-1-AP (SYTL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SYTL2 Polyclonal antibody (12359-1-AP)

    IHC staining of human breast cancer using 12359-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12359-1-AP (SYTL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SYTL2 Polyclonal antibody (12359-1-AP)

    IHC staining of human breast cancer using 12359-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12359-1-AP (SYTL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12359-1-AP targets SYTL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYTL2 fusion protein Ag3023 相同性解析による交差性が予測される生物種
Full Name synaptotagmin-like 2
Calculated molecular weight 934 aa, 105 kDa
Observed molecular weight39 kDa, 100-105 kDa
GenBank accession numberBC015540
Gene symbol SYTL2
Gene ID (NCBI) 54843
RRIDAB_11182492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptotagmin-like 2 (SYTL2) is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. SYTL2 contains an N-terminal Slp homology domain (SHD), Rab-binding region, and C-terminal tandem C2 domains, C2A and C2B (PMID: 11327731). It interacts with RAB27A and plays a role in promoting docking of RAB27-bound organelles to the plasma membrane (PMID: 17052176). Alternative splicing of SYTL2 gene results in multiple transcript variants encoding distinct isoforms with calculated molecular weights ranging from 39 kDa to 142 kDa.

Protocols

Product Specific Protocols
WB protocol for SYTL2 antibody 12359-1-APDownload protocol
IHC protocol for SYTL2 antibody 12359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Suppression of PD‐L1 release from small extracellular vesicles promotes systemic anti‐tumor immunity by targeting ORAI1 calcium channels

Authors - Xi Chen
  • KD Validated
View Article
humanIHC

Nat Commun

ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma

Authors - Miwa Tanaka
View Article