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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SYTL4 Recombinant antibody

SYTL4 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240556E2

Cat no : 83564-5-RR

Synonyms

synaptotagmin like 4, SLP4, Granuphilin, Exophilin-2, Exophilin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYTL4 Recombinant antibody (83564-5-RR)

    WB analysis using 83564-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83564-5-RR (SYTL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83564-5-RR

    Affinity and Kinetic Characterization of 83564-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83564-5-RR against Human SYTL4 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83564-5-RR is SYTL4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SYTL4 fusion protein Ag2772 相同性解析による交差性が予測される生物種
Full Name synaptotagmin-like 4
Calculated molecular weight 671 aa, 76 kDa
Observed molecular weight74-76 kDa
GenBank accession numberBC014913
Gene symbol SYTL4
Gene ID (NCBI) 94121
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)

Protocols

Product Specific Protocols
WB protocol for SYTL4 antibody 83564-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols