Featured Product
KD/KO Validated

HRD1/SYVN1 Polyclonal antibody

Name: HRD1/SYVN1 Polyclonal antibody
Brand: Proteintech
SKU: 13473-1-AP

HRD1/SYVN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13473-1-AP

Synonyms

SYVN1, Synovial apoptosis inhibitor 1, KIAA1810, HRD1, EC:2.3.2.27

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 13473-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13473-1-AP
WB result of HRD1/SYVN1 antibody (13473-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRD1/SYVN1 transfected HeLa cells.
WB analysis of Raji using 13473-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 13473-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 13473-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 13473-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13473-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 13473-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat stomach using 13473-1-AP
rat stomach tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse stomach using 13473-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13473-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 13473-1-AP
IP result of anti-HRD1/SYVN1 (IP:13473-1-AP, 4ug; Detection:13473-1-AP 1:1000) with mouse kidney tissue lysate 7200ug.
IHC staining of human kidney using 13473-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13473-1-AP (SYVN1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 13473-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13473-1-AP (SYVN1 antibody at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 13473-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HRD1/SYVN1 antibody (13473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 13473-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, MDA-MB-453s cells, mouse kidney tissue, mouse ovary tissue, mouse pancreas tissue, mouse stomach tissue, NIH/3T3 cells, Raji cells, rat stomach tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 14 publications below
WB	See 59 publications below
IHC	See 3 publications below
IF	See 7 publications below
IP	See 6 publications below
CoIP	See 5 publications below

Product Information

13473-1-AP targets HRD1/SYVN1 in WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HRD1/SYVN1 fusion protein Ag4267 相同性解析による交差性が予測される生物種
Full Name	synovial apoptosis inhibitor 1, synoviolin
Calculated molecular weight	617 aa, 68 kDa
Observed molecular weight	68-76 kDa
GenBank accession number	BC030530
Gene symbol	HRD1
Gene ID (NCBI)	84447
RRID	AB_2287023
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

Protocols

Product Specific Protocols
WB protocol for HRD1/SYVN1 antibody 13473-1-AP	Download protocol
IHC protocol for HRD1/SYVN1 antibody 13473-1-AP	Download protocol
IF protocol for HRD1/SYVN1 antibody 13473-1-AP	Download protocol
IP protocol for HRD1/SYVN1 antibody 13473-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. Authors - Hui Luo View Article
human	IP,WB	J Clin Invest Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders Authors - Huilun Helen Wang View Article
human	WB,IF,IP,IP-MS	Mol Cancer CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Authors - Ya Fan View Article
human	WB	Mol Cell Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation. Authors - Annemieke T van der Goot KD Validated View Article
		Mol Cell Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates. Authors - Corey N Cunningham View Article
human	WB	Nat Commun PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones. Authors - Yu-Jie Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HRD1/SYVN1 Polyclonal antibody

HRD1/SYVN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 13473-1-AP

WB analysis of HeLa using 13473-1-AP

WB analysis of Raji using 13473-1-AP

WB analysis of mouse pancreas using 13473-1-AP

WB analysis of MDA-MB-453s using 13473-1-AP

WB analysis of mouse ovary using 13473-1-AP

WB analysis of HepG2 using 13473-1-AP

WB analysis of NIH/3T3 using 13473-1-AP

WB analysis of rat stomach using 13473-1-AP

WB analysis of mouse stomach using 13473-1-AP

WB analysis of mouse kidney using 13473-1-AP

IP experiment of mouse kidney using 13473-1-AP

IHC staining of human kidney using 13473-1-AP

IHC staining of human kidney using 13473-1-AP

IF Staining of HepG2 using 13473-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.

Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.

PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones.