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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Sam50 Monoclonal antibody, PBS Only

Sam50 Monoclonal Antibody for IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H6H9

Cat no : 67425-1-PBS

Synonyms

SAMM50, SAMM 50, OMP85, CGI-51, CGI 51

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Sam50 Monoclonal antibody (67425-1-Ig)

    IF Staining of HeLa using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Sam50 Monoclonal antibody (67425-1-Ig)

    IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Sam50 Monoclonal antibody (67425-1-Ig)

    IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67425-1-PBS targets Sam50 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Sam50 fusion protein Ag15155 相同性解析による交差性が予測される生物種
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Calculated molecular weight 469 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007830
Gene symbol Sam50
Gene ID (NCBI) 25813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).