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  • Featured Product
  • KD/KO Validated

Septin 6 Polyclonal antibody, PBS Only

Septin 6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12805-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Septin 6 Polyclonal antibody (12805-1-AP)

    WB analysis of Jurkat using 12805-1-AP (same clone as 12805-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using Septin 6 Polyclonal antibody (12805-1-AP)

    WB analysis of K-562 using 12805-1-AP (same clone as 12805-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Septin 6 Polyclonal antibody (12805-1-AP)

    WB analysis of rat brain using 12805-1-AP (same clone as 12805-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using Septin 6 Polyclonal antibody (12805-1-AP)

    IP experiment of rat brain using 12805-1-AP (same clone as 12805-1-PBS)

    IP result of anti-Septin 6 (IP:12805-1-AP, 4ug; Detection:12805-1-AP 1:600) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Septin 6 Polyclonal antibody (12805-1-AP)

    IHC staining of human cervical cancer using 12805-1-AP (same clone as 12805-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (Septin 6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Septin 6 Polyclonal antibody (12805-1-AP)

    IHC staining of human cervical cancer using 12805-1-AP (same clone as 12805-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (SEPT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12805-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12805-1-PBS targets Septin 6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Septin 6 fusion protein Ag3521 相同性解析による交差性が予測される生物種
Full Name septin 6
Calculated molecular weight 434 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC036240
Gene symbol SEPT6
Gene ID (NCBI) 23157
RRIDAB_2184993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.