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  • Featured Product
  • KD/KO Validated

Septin 7 Monoclonal antibody, PBS Only

Septin 7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12D1

Cat no : 66542-1-PBS

Synonyms

septin7, SEPT7, SEPT 7, CDC3, CDC10 protein homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Septin 7 Monoclonal antibody (66542-1-Ig)

    WB analysis using 66542-1-Ig (same clone as 66542-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66542-1-Ig (Septin 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Septin 7 Monoclonal antibody (66542-1-Ig)

    WB analysis of HepG2 using 66542-1-Ig (same clone as 66542-1-PBS)

    WB result of Septin 7 antibody (66542-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 7 transfected HepG2 cells. This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Septin 7 Monoclonal antibody (66542-1-Ig)

    IHC staining of human gliomas using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Septin 7 Monoclonal antibody (66542-1-Ig)

    IHC staining of human gliomas using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Septin 7 Monoclonal antibody (66542-1-Ig)

    IHC staining of human lung using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Septin 7 Monoclonal antibody (66542-1-Ig)

    IHC staining of human tonsillitis using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Septin 7 Monoclonal antibody (66542-1-Ig)

    IHC staining of human tonsillitis using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Septin 7 Monoclonal antibody (66542-1-Ig)

    IHC staining of human tonsillitis using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Septin 7 Monoclonal antibody (66542-1-Ig)

    IHC staining of human lung using 66542-1-Ig (same clone as 66542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66542-1-Ig (Septin 7 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66542-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66542-1-PBS targets Septin 7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 7 fusion protein Ag4309 相同性解析による交差性が予測される生物種
Full Name septin 7
Calculated molecular weight 51 kDa
Observed molecular weight 48-51 kDa
GenBank accession numberBC025987
Gene symbol Septin 7
Gene ID (NCBI) 989
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. Septin 7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.