Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig colon tissue, A549 cells, rat colon tissue, MCF-7 cells, HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67548-1-Ig targets Septin 9 in WB, ELISA applications and shows reactivity with Human, pig, rat samples.
| Tested Reactivity | Human, pig, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Septin 9 fusion protein Ag18047 相同性解析による交差性が予測される生物種 |
| Full Name | septin 9 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC021192 |
| Gene Symbol | SEPT9 |
| Gene ID (NCBI) | 10801 |
| RRID | AB_2935251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UHD8 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 9 antibody 67548-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |