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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Serotonin transporter Recombinant antibody (84844-3-RR)

    WB analysis using 84844-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84844-3-RR (Serotonin transporter antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Serotonin transporter Recombinant antibody

Serotonin transporter Recombinant Antibody for WB, ELISA
Cat No. 84844-3-RR
Clone No.242252B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

SLC6A4, HTT, hSERT, 5HTT, 5 HTTLPR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, unboiled mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84844-3-RR targets Serotonin transporter in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Calculated molecular weight 70 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_001045
Gene Symbol Serotonin transporter
Gene ID (NCBI) 6532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31645
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, partly by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319).

Protocols

Product Specific Protocols
WB protocol for Serotonin transporter antibody 84844-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols