Serum amyloid P component Polyclonal antibody
Serum amyloid P component Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 31813-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human plasma |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
31813-1-AP targets Serum amyloid P component in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
Full Name | amyloid P component, serum |
Calculated molecular weight | 223 aa, 25 kDa |
Observed molecular weight | 27-29 kDa |
GenBank accession number | BC007058 |
Gene symbol | Serum amyloid P component |
Gene ID (NCBI) | 325 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Serum amyloid P component (SAP), a member of the pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand-binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Protocols
Product Specific Protocols | |
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WB protocol for Serum amyloid P component antibody 31813-1-AP | Download protocol |
IHC protocol for Serum amyloid P component antibody 31813-1-AP | Download protocol |
IF protocol for Serum amyloid P component antibody 31813-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |