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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Shh Recombinant antibody

Shh Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240057E7

Cat no : 83280-1-RR

Synonyms

M100081, Hxl3, Hx, Hhg1, Dsh

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using mouse Shh Recombinant antibody (83280-1-RR)

    WB analysis using 83280-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83280-1-RR

    Affinity and Kinetic Characterization of 83280-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83280-1-RR against Mouse SHH were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83280-1-RR is Shh Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name sonic hedgehog
Calculated molecular weight48KD
Observed molecular weight19-50 kDa
GenBank accession numberNM_009170.3
Gene symbol Shh
Gene ID (NCBI) 20423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Shh antibody 83280-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols