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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Siglec-7/CD328 Recombinant antibody, PBS Only

Siglec-7/CD328 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241525G3

Cat no : 84253-4-PBS

Synonyms

SIGLEC7, Siglec-7, D siglec, CD328, AIRM-1

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using Siglec-7/CD328 Recombinant antibody (84253-4-RR)

    WB analysis of U-937 using 84253-4-RR (same clone as 84253-4-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84253-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Siglec-7/CD328 Recombinant antibody (84253-4-RR)

    IHC staining of human tonsillitis using 84253-4-RR (same clone as 84253-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84253-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Siglec-7/CD328 Recombinant antibody (84253-4-RR)

    IHC staining of human tonsillitis using 84253-4-RR (same clone as 84253-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84253-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84253-4-RR

    Affinity and Kinetic Characterization of 84253-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84253-4-RR against Human Siglec-7/CD328 were performed. The affinity constant is 53.4 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84253-4-PBS targets Siglec-7/CD328 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name sialic acid binding Ig-like lectin 7
Calculated molecular weight51 kDa
Observed molecular weight75 kDa
GenBank accession numberNM_014385.4
Gene symbol Siglec-7
Gene ID (NCBI) 27036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).