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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Siglec-9 Recombinant antibody (84683-4-RR)

    WB analysis using 84683-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84683-4-RR (Siglec-9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84683-4-RR

    Affinity and Kinetic Characterization of 84683-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84683-4-RR against Human Siglec-9 were performed. The affinity constant is 0.492 nM.

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Siglec-9 Recombinant antibody

Siglec-9 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84683-4-RR
Clone No.241799E8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SIGLEC9, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9, CD329

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84683-4-RR targets Siglec-9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name sialic acid binding Ig-like lectin 9
Calculated molecular weight50 kDa
Observed molecular weight50 kDa
GenBank accession numberNM_014441.3
Gene Symbol Siglec-9
Gene ID (NCBI) 27180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y336-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

Protocols

Product Specific Protocols
WB protocol for Siglec-9 antibody 84683-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols