SignalBright Max Chemiluminescent Substrate

Product Information

SignalBright Max is a luminol-based, ultra-sensitive chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Max provides increased sensitivity over SignalBright Pro, with mid to low femtogram detection levels in Western Blot and ELISA analysis.

Key Characteristics:

· Ultra-Sensitivity: Mid to low femtogram

· Signal duration: > 12 hours

· Compatibility: suitable for PVDF and nitrocellulose membranes

· Comparable sensitivity to SuperSignal™ West Femto

Instructions for Use

SignalBright Max Chemiluminescent Detection Kit is prepared by combining a 1:1 ratio of Chemiluminescent Substrate Solution and  Stable Peroxide Solution to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm². Leave 5 minutes for signal to develop prior to imaging. Signal can be imaged using an X-ray film or a Chemiluminescent Imager.

· Recommended primary antibody concentration: 10-200 ng/ml

· Recommended HRP-conjugated secondary antibody concentration: 1-10 ng/ml