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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Snf1lk Recombinant antibody (82851-3-RR)

    WB analysis using 82851-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82851-3-RR (Snf1lk antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82851-3-RR

    Affinity and Kinetic Characterization of 82851-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82851-3-RR against Mouse Snf1lk were performed. The affinity constant is 0.686 nM.

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Snf1lk Recombinant antibody

Snf1lk Recombinant Antibody for WB, ELISA
Cat No. 82851-3-RR
Clone No.3D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Sik1, Salt-inducible kinase 1, salt inducible kinase 1, Myocardial SNF1-like kinase, Msk

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82851-3-RR targets Snf1lk in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Snf1lk fusion protein Ag33435 相同性解析による交差性が予測される生物種
Full Name salt inducible kinase 1
Calculated molecular weight 85 kDa
Observed molecular weight85 kDa
GenBank accession numberNM_010831
Gene Symbol Sik1
Gene ID (NCBI) 17691
RRIDAB_3670577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ60670
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Snf1lk antibody 82851-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols