Sortilin Monoclonal antibody
Sortilin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2B11B9
Cat no : 68007-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | ROS1728 cells, SK-N-SH cells, SH-SY5Y cells, pig brain tissue, rabbit brain, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68007-1-Ig targets Sortilin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Sortilin fusion protein Ag28043 相同性解析による交差性が予測される生物種 |
| Full Name | sortilin 1 |
| Calculated molecular weight | 831 aa, 92 kDa |
| Observed molecular weight | 95-100 kDa |
| GenBank accession number | BC023542 |
| Gene symbol | Sortilin |
| Gene ID (NCBI) | 6272 |
| RRID | AB_2918754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Sortilin antibody 68007-1-Ig | Download protocol |
| IHC protocol for Sortilin antibody 68007-1-Ig | Download protocol |
| IF protocol for Sortilin antibody 68007-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
