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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Spermidine synthase Monoclonal antibody, PBS Only

Spermidine synthase Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F4E10

Cat no : 68548-1-PBS

Synonyms

PAPT, SPDSY, spermidine synthase, SPS1, SRM, SRML1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Spermidine synthase Monoclonal antibody (68548-1-Ig)

    WB analysis using 68548-1-Ig (same clone as 68548-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68548-1-Ig (Spermidine synthase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68548-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using Spermidine synthase Monoclonal antibody (68548-1-Ig)

    IF Staining of RAW 264.7 using 68548-1-Ig (same clone as 68548-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Spermidine synthase antibody (68548-1-Ig, Clone: 2F4E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68548-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68548-1-PBS targets Spermidine synthase in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Spermidine synthase fusion protein Ag33919 相同性解析による交差性が予測される生物種
Full Name spermidine synthase
Calculated molecular weight 302 aa, 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC000309
Gene symbol Spermidine synthase
Gene ID (NCBI) 6723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).