ChromoTek Spot-Cap®
The ChromoTek Spot-Cap™ consists of an anti-Spot-tag® Nanobody (VHH), which is covalently bound to agarose. Spot-Cap is used to purify Spot-tagged proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. Spot-Cap is engineered for gentle elution with Spot-peptide at 4°C and high yields in batch or gravity flow formats.
Cat no : eca
Synonyms
Validation Data Gallery
Product Information
The ChromoTek Spot-Cap™ consists of an anti-Spot-tag® Nanobody (VHH), which is covalently bound to agarose. Spot-Cap is used to purify Spot-tagged proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. Spot-Cap is engineered for gentle elution with Spot-peptide at 4°C and high yields in batch or gravity flow formats.
| Description | Spot-Cap® is a Nanobody-based affinity resin optimized for one-step protein purification of Spot-tagged proteins:
• Economic: high binding capacity and multiple regeneration cycles • Fast and gentle one-step purification at 4°C preserves protein stability and function • Tight and highly selective binding of a single repeat Spot-Tag • High specificity leads to minimal contamination of host cell proteins • No contamination of antibody heavy and light chains |
| Applications | Protein purification |
| Specificity/Target | Spot-Tag® PDRVRAVSHWSS |
| Binding capacity | 10 mg protein (30 kDa) / 1 mL settled resin (340 nmol / 1 mL settled resin) |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | 0.1 mM Spot-peptide or 100 mM glycine pH 2.0 at 4°C |
| Wash buffer compatibility | 1 M NaCl, 2 M Urea, 10 mM DTT, 10 mM ß-mercaptoethanol, 10 mM TCEP, 2% DDM, 2% NP-40, 2% Triton X-100 |
| pH range for purification | pH 4 - 11 |
| Regeneration buffer | 100 mM glycine pH 2.0 |
| Regeneration | >5 cycles |
| Purification format | Batch and gravity flow |
| Affinity (KD) | Dissociation constant KD of 40 nM |
| RRID | AB_2827549 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Do not freeze! Stable for one year. |
| Spot-Tag® Origin | Engineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin |
| Cross Reactivity | The Spot-Nanobody shows minimal cross reactivity with beta-catenin. |
Publications
| Application | Title |
|---|---|
J Chromatogr A An engineered peptide tag-specific nanobody for immunoaffinity chromatography application enabling efficient product recovery at mild conditions. |