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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Stathmin 1 Polyclonal antibody (11157-1-AP)

    WB analysis of Jurkat using 11157-1-AP (same clone as 11157-1-PBS)

    WB results of Stathmin antibody (11157-1-AP, 1:20000) with si-control and si-Stathmin transfected Jurkat cells.. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Stathmin 1 Polyclonal antibody (11157-1-AP)

    WB analysis using 11157-1-AP (same clone as 11157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Stathmin 1 Polyclonal antibody (11157-1-AP)

    WB analysis of Jurkat using 11157-1-AP (same clone as 11157-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Stathmin 1 Polyclonal antibody (11157-1-AP)

    IHC staining of human gliomas using 11157-1-AP (same clone as 11157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11157-1-AP (Stathmin 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Stathmin 1 Polyclonal antibody (11157-1-AP)

    IHC staining of human gliomas using 11157-1-AP (same clone as 11157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Stathmin 1 Polyclonal antibody (11157-1-AP)

    IHC staining of human lymphoma using 11157-1-AP (same clone as 11157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Stathmin 1 Polyclonal antibody (11157-1-AP)

    IHC staining of human lymphoma using 11157-1-AP (same clone as 11157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Stathmin 1 Polyclonal antibody (11157-1-AP)

    IF Staining of HeLa using 11157-1-AP (same clone as 11157-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Stathmin 1 antibody (11157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Stathmin 1 Polyclonal antibody (11157-1-AP)

    FC experiment of HeLa using 11157-1-AP (same clone as 11157-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Stathmin 1 (11157-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Stathmin 1 Polyclonal antibody, PBS Only

Stathmin 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 11157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

STMN1, C1orf215, LAP18, Leukemia-associated phosphoprotein p18, OP18

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11157-1-PBS targets Stathmin 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Stathmin 1 fusion protein Ag1633 相同性解析による交差性が予測される生物種
Full Name stathmin 1/oncoprotein 18
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC014353
Gene Symbol Stathmin 1
Gene ID (NCBI) 3925
RRIDAB_2197114
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16949
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.