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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Stomatin Monoclonal antibody

Stomatin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4D6

Cat no : 67894-1-Ig

Synonyms

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Stomatin Monoclonal antibody (67894-1-Ig)

    WB analysis of human placenta using 67894-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using Stomatin Monoclonal antibody (67894-1-Ig)

    WB analysis of human testis using 67894-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Stomatin Monoclonal antibody (67894-1-Ig)

    WB analysis of HeLa using 67894-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using Stomatin Monoclonal antibody (67894-1-Ig)

    WB analysis of human peripheral blood platelets using 67894-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Stomatin Monoclonal antibody (67894-1-Ig)

    IHC staining of human lung cancer using 67894-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67894-1-Ig (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman placenta tissue, HeLa cells, human peripheral blood platelets, human testis tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67894-1-Ig targets Stomatin in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Stomatin fusion protein Ag2676 相同性解析による交差性が予測される生物種
Full Name stomatin
Calculated molecular weight 288 aa, 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC010703
Gene symbol Stomatin
Gene ID (NCBI) 2040
RRIDAB_2918650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Protocols

Product Specific Protocols
WB protocol for Stomatin antibody 67894-1-IgDownload protocol
IHC protocol for Stomatin antibody 67894-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols