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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptophysin Monoclonal antibody (67864-1-Ig)

    WB analysis using 67864-1-Ig (same clone as 67864-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Synaptophysin Monoclonal antibody (67864-1-Ig)

    WB analysis using 67864-1-Ig (same clone as 67864-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IHC staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IHC staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IHC staining of human rectal cancer using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IHC staining of rat pancreas using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IF Staining of mouse pancreas using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IF Staining of mouse brain using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IF Staining of mouse pancreas using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IF Staining of SH-SY5Y using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Synaptophysin Monoclonal antibody (67864-1-Ig)

    IF Staining of PC-12 using 67864-1-Ig (same clone as 67864-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67864-1-PBS in a different storage buffer formulation.

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Synaptophysin Monoclonal antibody, PBS Only

Synaptophysin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67864-1-PBS
Clone No.4E12C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

SYP, SYN, MRX96, 4E12C4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67864-1-PBS targets Synaptophysin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11803 相同性解析による交差性が予測される生物種
Full Name synaptophysin
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight38 kDa
GenBank accession numberBC064550
Gene Symbol Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2918622
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08247
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)