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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-1 Recombinant antibody

Synaptotagmin-1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240589F6

Cat no : 83637-4-RR

Synonyms

Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Synaptotagmin-1 Recombinant antibody (83637-4-RR)

    WB analysis using 83637-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83637-4-RR (Synaptotagmin-1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Synaptotagmin-1 Recombinant antibody (83637-4-RR)

    IF Staining of SH-SY5Y using 83637-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptotagmin-1 antibody (83637-4-RR, Clone: 240589F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Synaptotagmin-1 Recombinant antibody (83637-4-RR)

    IF Staining of SH-SY5Y using 83637-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptotagmin-1 antibody (83637-4-RR, Clone: 240589F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83637-4-RR

    Affinity and Kinetic Characterization of 83637-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83637-4-RR against Human Synaptotagmin-1 were performed. The affinity constant is 20.5 nM.

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Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83637-4-RR is Synaptotagmin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Synaptotagmin-1 fusion protein Ag5970 相同性解析による交差性が予測される生物種
Full Name synaptotagmin I
Calculated molecular weight 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC058917
Gene symbol Synaptotagmin-1
Gene ID (NCBI) 6857
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-1 antibody 83637-4-RRDownload protocol
IF protocol for Synaptotagmin-1 antibody 83637-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols