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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-3 Polyclonal antibody

Synaptotagmin-3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28667-1-AP

Synonyms

DKFZp761O132, Synaptotagmin 3, synaptotagmin III, SYT3, SytIII

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-3 Polyclonal antibody (28667-1-AP)

    WB analysis using 28667-1-AP

    mouse brain tissue and rat brain tissue were subjected to SDS PAGE followed by western blot with 28667-1-AP (Synaptotagmin-3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-3 Polyclonal antibody (28667-1-AP)

    IHC staining of mouse brain using 28667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28667-1-AP (Synaptotagmin-3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-3 Polyclonal antibody (28667-1-AP)

    IHC staining of mouse brain using 28667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28667-1-AP (Synaptotagmin-3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Synaptotagmin-3 Polyclonal antibody (28667-1-AP)

    IF Staining of mouse brain using 28667-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptotagmin-3 antibody (28667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28667-1-AP targets Synaptotagmin-3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptotagmin-3 fusion protein Ag30027 相同性解析による交差性が予測される生物種
Full Name synaptotagmin III
Calculated molecular weight 590 aa, 63 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC028379
Gene symbol Synaptotagmin-3
Gene ID (NCBI) 84258
RRIDAB_2918187
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-3 (SYT3) is highly expressed in brain, adipose tissue, and heart (PMID: 30545844). Synaptotagmin-3 is highly enriched in synaptic plasma membranes (PMID: 10373432). It has been reported that synaptotagmin-3 internalizes AMPA receptors to depress synaptic strength and promote forgetting (PMID: 30545844).

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-3 antibody 28667-1-APDownload protocol
IHC protocol for Synaptotagmin-3 antibody 28667-1-APDownload protocol
IF protocol for Synaptotagmin-3 antibody 28667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols