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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-8 Polyclonal antibody (20671-1-AP)

    WB analysis using 20671-1-AP (same clone as 20671-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20671-1-AP (Synaptotagmin-8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20671-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using Synaptotagmin-8 Polyclonal antibody (20671-1-AP)

    IF Staining of Caco-2 using 20671-1-AP (same clone as 20671-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Synaptotagmin-8 antibody (20671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20671-1-PBS in a different storage buffer formulation.

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Synaptotagmin-8 Polyclonal antibody, PBS Only

Synaptotagmin-8 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 20671-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20671-1-PBS targets Synaptotagmin-8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name synaptotagmin VIII
Calculated molecular weight 44 kDa
Observed molecular weight40 kDa, 50-54 kDa
GenBank accession numberNM_138567
Gene Symbol SYT8
Gene ID (NCBI) 90019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBV8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT8 (synaptotagmin-8) is related to insulin secretion, and its expression is positively regulated by the insulin promoter (PMID: 21336277). Due to alternative splicing, SYT8 may produce proteins with molecular weights between 25 and 40 to 44 kDa. An additional band of 50 kDa could also be detected (PMID: 16386321)