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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-9 Monoclonal antibody (68430-1-Ig)

    WB analysis using 68430-1-Ig (same clone as 68430-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68430-1-Ig (Synaptotagmin-9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68430-1-PBS in a different storage buffer formulation.

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Synaptotagmin-9 Monoclonal antibody, PBS Only

Synaptotagmin-9 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68430-1-PBS
Clone No.3D2F2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, Indirect ELISA

Synonyms

SytIX, SYT9, Synaptotagmin IX, 3D2F2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68430-1-PBS targets Synaptotagmin-9 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Synaptotagmin-9 fusion protein Ag33194 相同性解析による交差性が予測される生物種
Full Name synaptotagmin IX
Calculated molecular weight 491 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC029605
Gene Symbol Synaptotagmin-9
Gene ID (NCBI) 143425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86SS6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).