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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using Synaptotagmin-9 Recombinant antibody (84123-3-RR)

    WB analysis of mouse eye using 84123-3-RR (same clone as 84123-3-PBS)

    mouse eye tissue was subjected to SDS PAGE followed by western blot with 84123-3-RR (Synaptotagmin-9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84123-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse retina tissue using Synaptotagmin-9 Recombinant antibody (84123-3-RR)

    IF Staining of mouse retina using 84123-3-RR (same clone as 84123-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse retina tissue using Synaptotagmin-9 antibody (84123-3-RR, Clone: 241407A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84123-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84123-3-RR

    Affinity and Kinetic Characterization of 84123-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84123-3-RR against Human Synaptotagmin-9 were performed. The affinity constant is 22.5 nM.

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Synaptotagmin-9 Recombinant antibody, PBS Only

Synaptotagmin-9 Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 84123-3-PBS
Clone No.241407A11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

SytIX, SYT9, Synaptotagmin IX, 241407A11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84123-3-PBS targets Synaptotagmin-9 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Synaptotagmin-9 fusion protein Ag3520 相同性解析による交差性が予測される生物種
Full Name synaptotagmin IX
Calculated molecular weight 491 aa, 52 kDa
Observed molecular weight52-56 kDa
GenBank accession numberBC029605
Gene Symbol Synaptotagmin-9
Gene ID (NCBI) 143425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86SS6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).