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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    WB analysis using 27749-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27749-1-AP (Syndetin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    IP experiment of mouse brain using 27749-1-AP

    IP result of anti-Syndetin/CCDC132 (IP:27749-1-AP, 4ug; Detection:27749-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

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Syndetin/CCDC132 Polyclonal antibody

Syndetin/CCDC132 Polyclonal Antibody for WB, IP, ELISA
Cat No. 27749-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

CCDC132, EARP/GARPII complex subunit VPS50, Syndetin, Syndetin/CCDC132, VPS50

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27749-1-AP targets Syndetin/CCDC132 in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syndetin/CCDC132 fusion protein Ag26944 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 132
Calculated molecular weight 964 aa, 111 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC152424
Gene Symbol Syndetin/CCDC132
Gene ID (NCBI) 55610
RRIDAB_2880961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JG6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Syndetin/CCDC132 antibody 27749-1-APDownload protocol
IP protocol for Syndetin/CCDC132 antibody 27749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols