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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    WB analysis using 14259-1-AP (same clone as 14259-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    WB analysis of human heart using 14259-1-AP (same clone as 14259-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    WB analysis of human brain using 14259-1-AP (same clone as 14259-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    WB analysis of human heart using 14259-1-AP (same clone as 14259-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    WB analysis of mouse heart using 14259-1-AP (same clone as 14259-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    WB analysis of BxPC-3 using 14259-1-AP (same clone as 14259-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    WB analysis of mouse heart using 14259-1-AP (same clone as 14259-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IHC staining of human pancreas using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IHC staining of human pancreas using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IHC staining of mouse brain using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IHC staining of mouse brain using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IHC staining of human kidney using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IHC staining of human kidney using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IF Staining of HepG2 using 14259-1-AP (same clone as 14259-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Syntaxin 12 Polyclonal antibody (14259-1-AP)

    IF Staining of HepG2 using 14259-1-AP (same clone as 14259-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Syntaxin 12 Polyclonal antibody, PBS Only

Syntaxin 12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14259-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Syntaxin-12, STX12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14259-1-PBS targets Syntaxin 12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntaxin 12 fusion protein Ag5532 相同性解析による交差性が予測される生物種
Full Name syntaxin 12
Calculated molecular weight 32 kDa
Observed molecular weight 33-39 kDa, 66 kDa
GenBank accession numberBC046999
Gene Symbol Syntaxin 12
Gene ID (NCBI) 23673
RRIDAB_2198222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86Y82
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).