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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Syntaxin 16 Monoclonal antibody

Syntaxin 16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A9D8

Cat no : 66775-1-Ig

Synonyms

hsyn16, STX16, SYN16, syntaxin 16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    WB analysis using 66775-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    WB analysis of rat brain using 66775-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    WB analysis of mouse brain using 66775-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    IHC staining of human lung cancer using 66775-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    IHC staining of human lung cancer using 66775-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    IHC staining of human colon cancer using 66775-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    IHC staining of human colon cancer using 66775-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    IF Staining of HepG2 using 66775-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using Syntaxin 16 Monoclonal antibody (66775-1-Ig)

    IF Staining of Neuro-2a using 66775-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Syntaxin 16 antibody (66775-1-Ig, Clone: 4A9D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:100-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66775-1-Ig targets Syntaxin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntaxin 16 fusion protein Ag27926 相同性解析による交差性が予測される生物種
Full Name syntaxin 16
Calculated molecular weight 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC019042
Gene symbol Syntaxin 16
Gene ID (NCBI) 8675
RRIDAB_2882121
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 16 antibody 66775-1-IgDownload protocol
IHC protocol for Syntaxin 16 antibody 66775-1-IgDownload protocol
IF protocol for Syntaxin 16 antibody 66775-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols