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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    WB analysis of Y79 using 66437-1-Ig (same clone as 66437-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    WB analysis of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    WB analysis of fetal human brain using 66437-1-Ig (same clone as 66437-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    IHC staining of human brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    IHC staining of human brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Syntaxin 1A / Syntaxin 1B Monoclonal antibody (66437-1-Ig)

    IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Syntaxin 1A / Syntaxin 1B antibody (66437-1-Ig, Clone: 2D3B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Syntaxin 1A / Syntaxin 1B Monoclonal antibody, PBS Only

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66437-1-PBS
Clone No.2D3B5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66437-1-PBS targets Syntaxin 1A / Syntaxin 1B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

Tested Reactivity human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntaxin 1A / Syntaxin 1B fusion protein Ag10257 相同性解析による交差性が予測される生物種
Full Name syntaxin 1B
Calculated molecular weight 288 aa, 33 kDa
Observed molecular weight 33 kDa, 55 kDa
GenBank accession numberBC062298
Gene Symbol STX1B
Gene ID (NCBI) 112755
RRIDAB_2881807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61266
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).