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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Syntaxin 6 Monoclonal antibody, PBS Only

Syntaxin 6 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B6G4

Cat no : 60059-1-PBS

Synonyms

Syntaxin-6, STX6, 2B6G4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    WB analysis using 60059-1-Ig (same clone as 60059-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    WB analysis of Jurkat using 60059-1-Ig (same clone as 60059-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    WB analysis of SH-SY5Y using 60059-1-Ig (same clone as 60059-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    WB analysis of U-251 using 60059-1-Ig (same clone as 60059-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    IHC staining of human brain using 60059-1-Ig (same clone as 60059-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    IHC staining of human brain using 60059-1-Ig (same clone as 60059-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    IHC staining of human prostate cancer using 60059-1-Ig (same clone as 60059-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Syntaxin 6 Monoclonal antibody (60059-1-Ig)

    IHC staining of human prostate cancer using 60059-1-Ig (same clone as 60059-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60059-1-PBS targets Syntaxin 6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntaxin 6 fusion protein Ag1284 相同性解析による交差性が予測される生物種
Full Name syntaxin 6
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC009944
Gene symbol Syntaxin 6
Gene ID (NCBI) 10228
RRIDAB_2196504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.