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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

T7-tag Polyclonal antibody

T7-tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14441-1-AP

Synonyms

T7 tag, T7-tag

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using T7-tag Polyclonal antibody (14441-1-AP)

    WB analysis using 14441-1-AP

    T7-tagged fution protein(20ng) was subjected to SDS PAGE followed by western blot with 14441-1-AP (T7-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inRecombinant protein protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14441-1-AP targets T7-tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name T7-tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_2878060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The antibody is developed against a synthetic peptide MASMTGGQQMG, the T7 tag, which is present in many vectors.

Protocols

Product Specific Protocols
WB protocol for T7-tag antibody 14441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Adv

TRIM37 is a primate-specific E3 ligase for Huntingtin and accounts for the striatal degeneration in Huntington's disease

Authors - Yiyang Qin
View Article
WB

J Immunother Cancer

N-terminal acetylation of transcription factor LIP induces immune therapy resistance via suppression of PD-L1 expression in non-small cell lung cancer

Authors - Xiang He
View Article