Featured Product
KD/KO Validated

TAB1 Polyclonal antibody

Name: TAB1 Polyclonal antibody
Brand: Proteintech
SKU: 27566-1-AP

TAB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27566-1-AP

Synonyms

MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1

Validation Data Gallery

Filter:

AllWBIHC

WB analysis using 27566-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 27566-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 27566-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 27566-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 27566-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 27566-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 27566-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 27566-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 27566-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens).

-/ -

Cat No. 27566-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, HeLa cells, A375 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected in	human colon cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below
IF	See 1 publications below

Product Information

27566-1-AP targets TAB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TAB1 fusion protein Ag25576 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase kinase kinase 7 interacting protein 1
Calculated molecular weight	55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC050554
Gene symbol	TAB1
Gene ID (NCBI)	10454
RRID	AB_2880910
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TAB1 antibody 27566-1-AP	Download protocol
IHC protocol for TAB1 antibody 27566-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Theranostics Virus-induced p38 MAPK activation facilitates viral infection. Authors - Yuting Cheng KO Validated View Article
human	WB,IF	Int J Mol Sci MicroRNA-27a-5p Downregulates Expression of Proinflammatory Cytokines in Lipopolysaccharide-Stimulated Human Dental Pulp Cells via the NF-κB Signaling Pathway Authors - Shihan Wang KD Validated View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAB1 Polyclonal antibody

TAB1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 27566-1-AP

WB analysis of A375 using 27566-1-AP

WB analysis of K-562 using 27566-1-AP

WB analysis of NIH/3T3 using 27566-1-AP

WB analysis of HeLa using 27566-1-AP

WB analysis of HEK-293T using 27566-1-AP

IHC staining of human colon cancer using 27566-1-AP

IHC staining of human liver using 27566-1-AP