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  • Featured Product
  • KD/KO Validated

TAB1 Polyclonal antibody

TAB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27566-1-AP

Synonyms

MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAB1 Polyclonal antibody (27566-1-AP)

    WB analysis using 27566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TAB1 Polyclonal antibody (27566-1-AP)

    WB analysis of K-562 using 27566-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using TAB1 Polyclonal antibody (27566-1-AP)

    WB analysis of NIH/3T3 using 27566-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TAB1 Polyclonal antibody (27566-1-AP)

    WB analysis of HeLa using 27566-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using TAB1 Polyclonal antibody (27566-1-AP)

    WB analysis of HEK-293T using 27566-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using TAB1 Polyclonal antibody (27566-1-AP)

    WB analysis of A375 using 27566-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TAB1 Polyclonal antibody (27566-1-AP)

    IHC staining of human liver using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAB1 Polyclonal antibody (27566-1-AP)

    IHC staining of human colon cancer using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TAB1 Polyclonal antibody (27566-1-AP)

    IHC staining of human liver using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, A375 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27566-1-AP targets TAB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAB1 fusion protein Ag25576 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 7 interacting protein 1
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC050554
Gene symbol TAB1
Gene ID (NCBI) 10454
RRIDAB_2880910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TAB1 antibody 27566-1-APDownload protocol
IHC protocol for TAB1 antibody 27566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Virus-induced p38 MAPK activation facilitates viral infection.

Authors - Yuting Cheng
  • KO Validated
View Article