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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using TAC1 Polyclonal antibody (28599-1-AP)

    IHC staining of mouse brain using 28599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TAC1 Polyclonal antibody (28599-1-AP)

    IHC staining of mouse brain using 28599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hypothalamus tissue using TAC1 Polyclonal antibody (28599-1-AP)

    IHC staining of human hypothalamus using 28599-1-AP

    Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human hypothalamus tissue using TAC1 Polyclonal antibody (28599-1-AP)

    IHC staining of human hypothalamus using 28599-1-AP

    Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 40x lens)..

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TAC1 Polyclonal antibody (28599-1-AP)

    IF Staining of mouse brain using 28599-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TAC1 Polyclonal antibody (28599-1-AP)

    IF Staining of mouse brain using 28599-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using TAC1 Polyclonal antibody (28599-1-AP)

    IF Staining of PC-12 using 28599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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TAC1 Polyclonal antibody

TAC1 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 28599-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IF-P, ELISA

Synonyms

TACI, TAC 1, Protachykinin-1, Protachykinin 1, C-terminal-flanking peptide

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue, human hypothalamus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28599-1-AP targets TAC1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAC1 fusion protein Ag23477 相同性解析による交差性が予測される生物種
Full Name tachykinin, precursor 1
Calculated molecular weight 129 aa, 15 kDa
GenBank accession numberBC018047
Gene Symbol TAC1
Gene ID (NCBI) 6863
RRIDAB_2881178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20366
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAC1, also known as Protachykinin-1, is a gene that encodes for a family of neuropeptides known as tachykinins. These peptides are characterized by their ability to rapidly stimulate contraction of intestinal muscle, hence the name "tachykinins". The TAC1 gene and its products are widely distributed in the nervous system, with α-TAC1 mRNA localized in numerous neurons of the brain. β-TAC1 and γ-TAC1 are predominantly expressed in intrinsic enteric neurons and sensory neurons. Additionally, the expression of TAC1, SP, and NKA is observed in various peripheral neurons and non-neural tissues such as the salivary gland, heart, skin, spleen, adrenal gland, and more.

Protocols

Product Specific Protocols
IHC protocol for TAC1 antibody 28599-1-APDownload protocol
IF protocol for TAC1 antibody 28599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols