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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TACC1 Polyclonal antibody (13862-1-AP)

    WB analysis of MCF-7 using 13862-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13862-1-AP (TACC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TACC1 Polyclonal antibody (13862-1-AP)

    WB analysis of HeLa using 13862-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13862-1-AP (TACC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TACC1 Polyclonal antibody (13862-1-AP)

    IHC staining of human stomach cancer using 13862-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13862-1-AP (TACC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TACC1 Polyclonal antibody (13862-1-AP)

    IHC staining of human stomach cancer using 13862-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13862-1-AP (TACC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TACC1 Polyclonal antibody (13862-1-AP)

    IF Staining of HeLa using 13862-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TACC1 antibody (13862-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TACC1 Polyclonal antibody (13862-1-AP)

    IF Staining of HeLa using 13862-1-AP

    Immunofluorescent analysis of HeLa cells using 13862-1-AP (TACC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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TACC1 Polyclonal antibody

TACC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13862-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Transforming acidic coiled-coil-containing protein 1, Taxin-1, Taxin1, KIAA1103, Ga55

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13862-1-AP targets TACC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TACC1 fusion protein Ag4842 相同性解析による交差性が予測される生物種
Full Name transforming, acidic coiled-coil containing protein 1
Calculated molecular weight 805 aa, 88 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberBC041391
Gene Symbol TACC1
Gene ID (NCBI) 6867
RRIDAB_2255719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75410
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TACC1 antibody 13862-1-APDownload protocol
IHC protocol for TACC1 antibody 13862-1-APDownload protocol
IF protocol for TACC1 antibody 13862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Discov

Loss of TACC1 variant25 inducing cell proliferation and suppressing autophagy in head and neck squamous carcinoma.

Authors - Pan Xu
View Article