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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TACSTD2/TROP2 Polyclonal antibody

TACSTD2/TROP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29856-1-AP

Synonyms

TACSTD2, M1S1, GA733-1, GA733 1, GA733

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TACSTD2/TROP2 Polyclonal antibody (29856-1-AP)

    WB analysis using 29856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TACSTD2/TROP2 Polyclonal antibody (29856-1-AP)

    IHC staining of human skin cancer using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TACSTD2/TROP2 Polyclonal antibody (29856-1-AP)

    IHC staining of human skin cancer using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TACSTD2/TROP2 Polyclonal antibody (29856-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TACSTD2/TROP2 Polyclonal antibody (29856-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TACSTD2/TROP2 Polyclonal antibody (29856-1-AP)

    IHC staining of human ovary cancer using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TACSTD2/TROP2 Polyclonal antibody (29856-1-AP)

    IHC staining of human ovary cancer using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using TACSTD2/TROP2 Polyclonal antibody (29856-1-AP)

    IF Staining of HaCaT using 29856-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (29856-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HaCaT cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29856-1-AP targets TACSTD2/TROP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TACSTD2/TROP2 fusion protein Ag31473 相同性解析による交差性が予測される生物種
Full Name tumor-associated calcium signal transducer 2
Calculated molecular weight 36 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC009409
Gene symbol TROP2
Gene ID (NCBI) 4070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).

Protocols

Product Specific Protocols
WB protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
IHC protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
IF protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols