Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
11299-1-AP targets TADA2L in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TADA2L fusion protein Ag1817 相同性解析による交差性が予測される生物種 |
| Full Name | transcriptional adaptor 2 (ADA2 homolog, yeast)-like |
| Calculated molecular weight | 51 aa, 5 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC011753 |
| Gene Symbol | TADA2L |
| Gene ID (NCBI) | 6871 |
| RRID | AB_717042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75478 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TADA2A is a subunit of the ADA2A-containing (ATAC) histone acetyltransferase complex, which with histone acetyltransferase activity on histones H3 and H4. It is required for the function of some acidic activation domains, which activate transcription from a distant site. It has a role in chromatin remodeling by binding at the linker region between neighbouring unclesomes of dinucleosomes [PMID:19103755].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TADA2L antibody 11299-1-AP | Download protocol |
| IHC protocol for TADA2L antibody 11299-1-AP | Download protocol |
| IP protocol for TADA2L antibody 11299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Biol The double-histone-acetyltransferase complex ATAC is essential for mammalian development. |