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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of Jurkat using 25521-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of HEK-293 using 25521-1-AP

    WB result of TAF15 antibody (25521-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HEK-293 cells.

  • Western Blot (WB) analysis of SH-SY5Y cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of SH-SY5Y using 25521-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of A549 using 25521-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of mouse brain using 25521-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of HEK-293 using 25521-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of HeLa using 25521-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human kidney using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25521-1-AP (TAF15 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human malignant melanoma using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human cervical cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human kidney using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25521-1-AP (TAF15 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

TAF15 Polyclonal antibody

TAF15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25521-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human kidney tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25521-1-AP targets TAF15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Calculated molecular weight 62 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberNM_003487
Gene Symbol TAF15
Gene ID (NCBI) 8148
RRIDAB_2880116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92804
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TAF15 antibody 25521-1-APDownload protocol
IHC protocol for TAF15 antibody 25521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

TFII-I/GTF2I regulates globin gene expression and stress response in erythroid cells

Authors - Rukiye Nar
View Article