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  • Featured Product
  • KD/KO Validated

TAF15 Polyclonal antibody

TAF15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25521-1-AP

Synonyms

TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of Jurkat using 25521-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of HEK-293 using 25521-1-AP

    WB result of TAF15 antibody (25521-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HEK-293 cells.

  • Western Blot (WB) analysis of SH-SY5Y cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of SH-SY5Y using 25521-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of A549 using 25521-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of mouse brain using 25521-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of HEK-293 using 25521-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TAF15 Polyclonal antibody (25521-1-AP)

    WB analysis of HeLa using 25521-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human kidney using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25521-1-AP (TAF15 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human malignant melanoma using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human cervical cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human kidney using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25521-1-AP (TAF15 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TAF15 Polyclonal antibody (25521-1-AP)

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human kidney tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25521-1-AP targets TAF15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Calculated molecular weight 62 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberNM_003487
Gene symbol TAF15
Gene ID (NCBI) 8148
RRIDAB_2880116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TAF15 antibody 25521-1-APDownload protocol
IHC protocol for TAF15 antibody 25521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Manohar (Verified Customer) (05-13-2020)

There are multiple bands appearing on western blot. When used with 5% blocking milk and 1:1000 antibody dilution

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T