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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAF15 Recombinant antibody, PBS Only

TAF15 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3L13

Cat no : 82860-1-PBS

Synonyms

RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68, 68 kDa TATA-binding protein-associated factor

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TAF15 Recombinant antibody (82860-1-RR)

    WB analysis of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    WB result of TAF15 antibody (82860-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HeLa cells. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TAF15 Recombinant antibody (82860-1-RR)

    WB analysis using 82860-1-RR (same clone as 82860-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82860-1-RR (TAF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TAF15 Recombinant antibody (82860-1-RR)

    WB analysis of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82860-1-RR (TAF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TAF15 Recombinant antibody (82860-1-RR)

    IF Staining of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TAF15 antibody (82860-1-RR, Clone: 3L13 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).CoraLite® Plus 488-Phalloidin (green) This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82860-1-PBS

    Affinity and Kinetic Characterization of 82860-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82860-1-RR against Human TAF15 were performed. The affinity constant is 0.974 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82860-1-PBS targets TAF15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TAF15 fusion protein Ag20828 相同性解析による交差性が予測される生物種
Full Name TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Calculated molecular weight 592 aa, 62 kDa
Observed molecular weight62-75 kDa
GenBank accession numberBC046099
Gene symbol TAF15
Gene ID (NCBI) 8148
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).