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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAF1C Polyclonal antibody

TAF1C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13341-1-AP

Synonyms

SL1, TAF1C, TAFI110, TAFI95, TBP associated factor 1C

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using TAF1C Polyclonal antibody (13341-1-AP)

    WB analysis of HT-1080 using 13341-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 13341-1-AP (TAF1C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TAF1C Polyclonal antibody (13341-1-AP)

    IHC staining of human liver cancer using 13341-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13341-1-AP (TAF1C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TAF1C Polyclonal antibody (13341-1-AP)

    IHC staining of human liver cancer using 13341-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13341-1-AP (TAF1C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHT-1080 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13341-1-AP targets TAF1C in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAF1C fusion protein Ag4164 相同性解析による交差性が予測される生物種
Full Name TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa
Calculated molecular weight 869 aa, 95 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC028131
Gene symbol TAF1C
Gene ID (NCBI) 9013
RRIDAB_10646433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TAF1C antibody 13341-1-APDownload protocol
IHC protocol for TAF1C antibody 13341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols