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Validation Data Gallery

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  • Western Blot (WB) analysis of cells using TAF3 Polyclonal antibody (18901-1-AP)

    WB analysis of cells using 18901-1-AP

    WB results of TAF3 (18901-1-AP) with siRNA sample. (140 kDa band absent in siRNA sample).

  • Western Blot (WB) analysis of various lysates using TAF3 Polyclonal antibody (18901-1-AP)

    WB analysis using 18901-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18901-1-AP (TAF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

TAF3 Polyclonal antibody

TAF3 Polyclonal Antibody for WB, ELISA
Cat No. 18901-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TAFII-140, TAFII140, TAFII 140, TAF140, TAF(II)140

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, cells, SW480 cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18901-1-AP targets TAF3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAF3 fusion protein Ag13536 相同性解析による交差性が予測される生物種
Full Name TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
Calculated molecular weight 929 aa, 104 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC024661
Gene Symbol TAF3
Gene ID (NCBI) 83860
RRIDAB_2878567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VWG9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAF3, also named as TAF140, is a 140 kDa subunit of Transcription initiation factor TFIID. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF3 has some isoforms with 727aa (~80 kDa), 622aa and 929aa (105 kDa).

Protocols

Product Specific Protocols
WB protocol for TAF3 antibody 18901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

MBio

A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression.

Authors - Hyung Suk Oh
View Article