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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAF5L Polyclonal antibody

TAF5L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19274-1-AP

Synonyms

TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PCAF associated factor 65 beta, PAF65-beta, PAF65B

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TAF5L Polyclonal antibody (19274-1-AP)

    WB analysis of Jurkat using 19274-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19274-1-AP (TAF5L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TAF5L Polyclonal antibody (19274-1-AP)

    IHC staining of human ovary cancer using 19274-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19274-1-AP (TAF5L antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19274-1-AP targets TAF5L in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAF5L fusion protein Ag5942 相同性解析による交差性が予測される生物種
Full Name TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC041094
Gene symbol TAF5L
Gene ID (NCBI) 27097
RRIDAB_10642700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TAF5L antibody 19274-1-APDownload protocol
IHC protocol for TAF5L antibody 19274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Nat Struct Mol Biol

A cohesin traffic pattern genetically linked to gene regulation

Authors - Anne-Laure Valton
View Article
humanWB

Sci Adv

The KAT module of the SAGA complex maintains the oncogenic gene expression program in MYCN-amplified neuroblastoma

Authors - Clare F Malone
View Article