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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAF6L Monoclonal antibody

TAF6L Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5C4

Cat no : 67569-1-Ig

Synonyms

FLJ11136, PAF65 alpha, PAF65A, TAF6L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAF6L Monoclonal antibody (67569-1-Ig)

    WB analysis using 67569-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67569-1-Ig (TAF6L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TAF6L Monoclonal antibody (67569-1-Ig)

    IHC staining of human breast cancer using 67569-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67569-1-Ig (TAF6L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TAF6L Monoclonal antibody (67569-1-Ig)

    IHC staining of human breast cancer using 67569-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67569-1-Ig (TAF6L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67569-1-Ig targets TAF6L in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TAF6L fusion protein Ag8202 相同性解析による交差性が予測される生物種
Full Name TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
Calculated molecular weight 622 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC008785
Gene symbol TAF6L
Gene ID (NCBI) 10629
RRIDAB_2882783
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TAF6L antibody 67569-1-IgDownload protocol
IHC protocol for TAF6L antibody 67569-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols