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KD/KO Validated

TAF9 Polyclonal antibody

Name: TAF9 Polyclonal antibody
Brand: Proteintech
SKU: 10544-1-AP
Rating: 5.00 (1 reviews)

TAF9 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 10544-1-AP

Synonyms

AD 004, AK6, CGI 137, CINAP, CIP, STAF31/32, TAF2G, TAF9, TAFII 31, TAFII 32, TAFII31, TAFII32, TAFIID32

Validation Data Gallery

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AllWBIP

WB analysis of HeLa using 10544-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10544-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10544-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10544-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10544-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10544-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 10544-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 10544-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10544-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 10544-1-AP
IP result of anti-TAF9 (IP:10544-1-AP, 4ug; Detection:10544-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

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Cat No. 10544-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, human colon tissue, human skeletal muscle tissue, HEK-293 cells, human heart tissue, rat liver tissue
Positive IP detected in	mouse skeletal muscle tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 2 publications below
IP	See 1 publications below

Product Information

10544-1-AP targets TAF9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TAF9 fusion protein Ag0833 相同性解析による交差性が予測される生物種
Full Name	TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa
Calculated molecular weight	28 kDa
Observed molecular weight	24-28 kDa
GenBank accession number	BC007426
Gene symbol	TAF9
Gene ID (NCBI)	6880
RRID	AB_2271416
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TAF9, also named as TAF2G, TAFII31, is a 264 amino acid protein, which belongs to the TAF9 family. TAF9 are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. TAF9 may have a role in gene regulation associated with apoptosis.

Protocols

Product Specific Protocols
WB protocol for TAF9 antibody 10544-1-AP	Download protocol
IP protocol for TAF9 antibody 10544-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cancers (Basel) Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models. Authors - Ha-Yeon Shin View Article
human	WB	Front Oncol Stable Isotope Tracing Uncovers Reduced γ/β-ATP Turnover and Metabolic Flux Through Mitochondrial-Linked Phosphotransfer Circuits in Aggressive Breast Cancer Cells. Authors - Aleksandr Klepinin View Article
mouse,rat	WB,IHC,IP	Front Pharmacol Activation of TAF9 via Danshensu-Induced Upregulation of HDAC1 Expression Alleviates Non-alcoholic Fatty Liver Disease. Authors - Ruiwen Wang KD Validated View Article
human	WB	J Proteome Res Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells. Authors - Weili Miao View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Aditya (Verified Customer) (10-08-2024)

good, very low on blot - convenient

Applications: Western Blot

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TAF9 Polyclonal antibody

TAF9 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 10544-1-AP

WB analysis of MCF-7 using 10544-1-AP

WB analysis of HEK-293 using 10544-1-AP

WB analysis of rat liver using 10544-1-AP

WB analysis of HeLa using 10544-1-AP

WB analysis of MCF-7 using 10544-1-AP

WB analysis of human colon using 10544-1-AP

WB analysis of human skeletal muscle using 10544-1-AP

WB analysis of human heart using 10544-1-AP

IP experiment of mouse skeletal muscle using 10544-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.

Stable Isotope Tracing Uncovers Reduced γ/β-ATP Turnover and Metabolic Flux Through Mitochondrial-Linked Phosphotransfer Circuits in Aggressive Breast Cancer Cells.

Activation of TAF9 via Danshensu-Induced Upregulation of HDAC1 Expression Alleviates Non-alcoholic Fatty Liver Disease.

Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells.

Reviews