TAF9B Polyclonal antibody
TAF9B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, IP, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 28713-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
28713-1-AP targets TAF9B in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TAF9B fusion protein Ag29633 相同性解析による交差性が予測される生物種 |
| Full Name | TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC010350 |
| Gene symbol | TAF9B |
| Gene ID (NCBI) | 51616 |
| RRID | AB_3086081 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transcription initiation factor TFIID subunit 9B (TAF9B), also names neuronal cell death-related protein 7, transcription initiation factor TFIID subunit 9-like, transcription-associated factor TAFII31L. It is essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes, may have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. The calculated MW of TAF9B is 28 kDa, 28713-1-AP can detect a band around 30 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF9B antibody 28713-1-AP | Download protocol |
| IHC protocol for TAF9B antibody 28713-1-AP | Download protocol |
| IP protocol for TAF9B antibody 28713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation |
